| # | Group A | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Sacramento Republic | 9 | 5 | 4 | 0 | 22:13 | 9 | 19 | |
| 2. | Reno | 8 | 5 | 2 | 1 | 22:11 | 11 | 17 | |
| 3. | Tacoma Defiance | 6 | 3 | 0 | 3 | 9:8 | 1 | 9 | |
| 4. | Portland Timbers 2 | 8 | 2 | 0 | 6 | 12:20 | -8 | 6 | |
| # | Group B | MP | W | D | L | G | GD | Pts | Form |
| 1. | Phoenix Rising | 9 | 8 | 0 | 1 | 32:9 | 23 | 24 | |
| 2. | Orange County SC | 8 | 4 | 3 | 1 | 7:4 | 3 | 15 | |
| 3. | Ventura County | 8 | 4 | 1 | 3 | 14:16 | -2 | 13 | |
| 4. | Las Vegas Lights | 8 | 2 | 3 | 3 | 14:15 | -1 | 9 | |
| 5. | San Diego Loyal | 8 | 2 | 2 | 4 | 6:11 | -5 | 8 | |
| # | Group C | MP | W | D | L | G | GD | Pts | Form |
| 1. | El Paso | 10 | 7 | 2 | 1 | 18:11 | 7 | 23 | |
| 2. | Colorado Springs | 10 | 1 | 6 | 3 | 10:12 | -2 | 9 | |
| 3. | Real Monarchs | 11 | 2 | 1 | 8 | 10:19 | -9 | 7 | |
| 4. | New Mexico | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 | |
| # | Group D | MP | W | D | L | G | GD | Pts | Form |
| 1. | San Antonio | 8 | 7 | 1 | 0 | 17:5 | 12 | 22 | |
| 2. | FC Tulsa | 8 | 4 | 3 | 1 | 12:8 | 4 | 15 | |
| 3. | Austin Bold | 8 | 4 | 2 | 2 | 17:12 | 5 | 14 | |
| 4. | Rio Grande | 7 | 2 | 1 | 4 | 12:16 | -4 | 7 | |
| 5. | Oklahoma City Energy | 8 | 0 | 5 | 3 | 4:11 | -7 | 5 | |
| # | Group E | MP | W | D | L | G | GD | Pts | Form |
| 1. | Louisville City | 11 | 7 | 1 | 3 | 20:10 | 10 | 22 | |
| 2. | Saint Louis FC | 10 | 6 | 2 | 2 | 18:12 | 6 | 20 | |
| 3. | Indy Eleven | 10 | 5 | 1 | 4 | 14:12 | 2 | 16 | |
| 4. | Sporting Kansas City II | 2 | 1 | 0 | 1 | 4:2 | 2 | 3 | |
| # | Group F | MP | W | D | L | G | GD | Pts | Form |
| 1. | Hartford Athletic | 9 | 6 | 2 | 1 | 20:13 | 7 | 20 | |
| 2. | Pittsburgh | 8 | 5 | 1 | 2 | 20:6 | 14 | 16 | |
| 3. | New York Red Bulls 2 | 9 | 3 | 0 | 6 | 14:17 | -3 | 9 | |
| 4. | Philadelphia Union II | 8 | 2 | 1 | 5 | 11:21 | -10 | 7 | |
| 5. | Loudoun | 4 | 0 | 1 | 3 | 3:7 | -4 | 1 | |
| # | Group G | MP | W | D | L | G | GD | Pts | Form |
| 1. | Charlotte Independ. | 8 | 3 | 3 | 2 | 11:12 | -1 | 12 | |
| 2. | Birmingham | 8 | 3 | 2 | 3 | 14:11 | 3 | 11 | |
| 3. | Memphis | 7 | 2 | 2 | 3 | 9:11 | -2 | 8 | |
| 4. | North Carolina | 8 | 2 | 0 | 6 | 6:13 | -7 | 6 | |
| # | Group H | MP | W | D | L | G | GD | Pts | Form |
| 1. | Charleston | 7 | 6 | 0 | 1 | 14:5 | 9 | 18 | |
| 2. | Tampa Bay | 8 | 5 | 2 | 1 | 15:7 | 8 | 17 | |
| 3. | Miami FC | 8 | 3 | 2 | 3 | 12:16 | -4 | 11 | |
| 4. | Atlanta United 2 | 8 | 2 | 2 | 4 | 14:16 | -2 | 8 |