| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Louisville City | 17 | 13 | 2 | 2 | 38:14 | 24 | 41 | |
| 2. | Tampa Bay | 17 | 12 | 3 | 2 | 41:13 | 28 | 39 | |
| 3. | Memphis | 17 | 11 | 1 | 5 | 40:22 | 18 | 34 | |
| 4. | Pittsburgh | 17 | 9 | 5 | 3 | 25:15 | 10 | 32 | |
| 5. | Birmingham | 17 | 9 | 4 | 4 | 29:15 | 14 | 31 | |
| 6. | FC Tulsa | 17 | 9 | 3 | 5 | 27:23 | 4 | 30 | |
| 7. | Indy Eleven | 17 | 9 | 2 | 6 | 26:22 | 4 | 29 | |
| 8. | Detroit | 17 | 6 | 8 | 3 | 21:16 | 5 | 26 | |
| 9. | Hartford Athletic | 17 | 7 | 1 | 9 | 28:28 | 0 | 22 | |
| 10. | Miami FC | 17 | 5 | 7 | 5 | 18:18 | 0 | 22 | |
| 11. | Loudoun | 17 | 6 | 2 | 9 | 22:30 | -8 | 20 | |
| 12. | Charleston | 17 | 5 | 2 | 10 | 23:35 | -12 | 17 | |
| 13. | Atlanta United 2 | 17 | 3 | 4 | 10 | 18:36 | -18 | 13 | |
| 14. | New York Red Bulls 2 | 17 | 2 | 5 | 10 | 14:33 | -19 | 11 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | San Antonio | 17 | 11 | 5 | 1 | 31:11 | 20 | 38 | |
| 2. | San Diego Loyal | 17 | 12 | 1 | 4 | 40:24 | 16 | 37 | |
| 3. | Sacramento Republic | 17 | 10 | 5 | 2 | 32:11 | 21 | 35 | |
| 4. | Colorado Springs | 17 | 10 | 3 | 4 | 35:24 | 11 | 33 | |
| 5. | Phoenix Rising | 17 | 9 | 3 | 5 | 32:21 | 11 | 30 | |
| 6. | Monterey Bay | 17 | 8 | 3 | 6 | 27:20 | 7 | 27 | |
| 7. | El Paso | 17 | 8 | 2 | 7 | 33:23 | 10 | 26 | |
| 8. | Rio Grande | 17 | 8 | 2 | 7 | 21:17 | 4 | 26 | |
| 9. | Oakland Roots | 17 | 7 | 5 | 5 | 26:22 | 4 | 26 | |
| 10. | Ventura County | 17 | 7 | 4 | 6 | 33:32 | 1 | 25 | |
| 11. | Las Vegas Lights | 17 | 7 | 3 | 7 | 23:23 | 0 | 24 | |
| 12. | New Mexico | 17 | 6 | 5 | 6 | 25:20 | 5 | 23 | |
| 13. | Orange County SC | 17 | 5 | 8 | 4 | 28:24 | 4 | 23 |