| # | Group A | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Reno | 8 | 6 | 1 | 1 | 21:10 | 11 | 19 | |
| 2. | Sacramento Republic | 7 | 3 | 2 | 2 | 5:4 | 1 | 11 | |
| 3. | Tacoma Defiance | 10 | 1 | 2 | 7 | 16:24 | -8 | 5 | |
| 4. | Portland Timbers 2 | 8 | 1 | 0 | 7 | 8:30 | -22 | 3 | |
| # | Group B | MP | W | D | L | G | GD | Pts | Form |
| 1. | San Diego Loyal | 8 | 4 | 3 | 1 | 11:7 | 4 | 15 | |
| 2. | Ventura County | 8 | 4 | 1 | 3 | 15:16 | -1 | 13 | |
| 3. | Phoenix Rising | 7 | 3 | 2 | 2 | 14:8 | 6 | 11 | |
| 4. | Orange County SC | 8 | 3 | 0 | 5 | 11:14 | -3 | 9 | |
| 5. | Las Vegas Lights | 8 | 0 | 2 | 6 | 10:19 | -9 | 2 | |
| # | Group C | MP | W | D | L | G | GD | Pts | Form |
| 1. | New Mexico | 15 | 8 | 3 | 4 | 23:17 | 6 | 27 | |
| 2. | El Paso | 6 | 2 | 3 | 1 | 6:3 | 3 | 9 | |
| 3. | Real Monarchs | 5 | 1 | 1 | 3 | 4:6 | -2 | 4 | |
| 4. | Colorado Springs | 6 | 1 | 1 | 4 | 9:16 | -7 | 4 | |
| # | Group D | MP | W | D | L | G | GD | Pts | Form |
| 1. | San Antonio | 8 | 3 | 2 | 3 | 13:9 | 4 | 11 | |
| 2. | FC Tulsa | 7 | 2 | 4 | 1 | 9:8 | 1 | 10 | |
| 3. | Austin Bold | 8 | 1 | 5 | 2 | 13:15 | -2 | 8 | |
| 4. | Oklahoma City Energy | 8 | 1 | 2 | 5 | 8:18 | -10 | 5 | |
| 5. | Rio Grande | 7 | 0 | 2 | 5 | 5:12 | -7 | 2 | |
| # | Group E | MP | W | D | L | G | GD | Pts | Form |
| 1. | Louisville City | 5 | 4 | 1 | 0 | 8:2 | 6 | 13 | |
| 2. | Sporting Kansas City II | 14 | 4 | 1 | 9 | 17:28 | -11 | 13 | |
| 3. | Indy Eleven | 6 | 2 | 1 | 3 | 7:7 | 0 | 7 | |
| 4. | Saint Louis FC | 6 | 1 | 2 | 3 | 4:9 | -5 | 5 | |
| # | Group F | MP | W | D | L | G | GD | Pts | Form |
| 1. | Pittsburgh | 8 | 6 | 0 | 2 | 19:4 | 15 | 18 | |
| 2. | Hartford Athletic | 7 | 5 | 0 | 2 | 11:11 | 0 | 15 | |
| 3. | New York Red Bulls 2 | 7 | 2 | 0 | 5 | 16:20 | -4 | 6 | |
| 4. | Loudoun | 9 | 1 | 2 | 6 | 7:21 | -14 | 5 | |
| 5. | Philadelphia Union II | 8 | 0 | 2 | 6 | 9:24 | -15 | 2 | |
| # | Group G | MP | W | D | L | G | GD | Pts | Form |
| 1. | Charlotte Independ. | 8 | 5 | 1 | 2 | 13:10 | 3 | 16 | |
| 2. | Birmingham | 8 | 4 | 2 | 2 | 15:8 | 7 | 14 | |
| 3. | North Carolina | 7 | 4 | 1 | 2 | 11:8 | 3 | 13 | |
| 4. | Memphis | 8 | 2 | 2 | 4 | 15:20 | -5 | 8 | |
| # | Group H | MP | W | D | L | G | GD | Pts | Form |
| 1. | Tampa Bay | 8 | 5 | 1 | 2 | 10:4 | 6 | 16 | |
| 2. | Charleston | 8 | 3 | 3 | 2 | 12:10 | 2 | 12 | |
| 3. | Miami FC | 8 | 1 | 2 | 5 | 8:18 | -10 | 5 | |
| 4. | Atlanta United 2 | 8 | 1 | 1 | 6 | 9:17 | -8 | 4 |