| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Nashville SC | 17 | 10 | 3 | 4 | 29:15 | 14 | 33 | |
| 2. | Pittsburgh | 17 | 9 | 4 | 4 | 28:20 | 8 | 31 | |
| 3. | Tampa Bay | 17 | 8 | 4 | 5 | 28:19 | 9 | 28 | |
| 4. | Louisville City | 17 | 7 | 5 | 5 | 20:20 | 0 | 26 | |
| 5. | New York Red Bulls 2 | 17 | 6 | 3 | 8 | 32:36 | -4 | 21 | |
| 6. | Indy Eleven | 17 | 6 | 2 | 9 | 22:23 | -1 | 20 | |
| 7. | Birmingham | 17 | 5 | 4 | 8 | 22:36 | -14 | 19 | |
| 8. | North Carolina | 17 | 5 | 3 | 9 | 17:22 | -5 | 18 | |
| 9. | Ottawa Fury | 17 | 4 | 5 | 8 | 22:27 | -5 | 17 | |
| 10. | Memphis | 17 | 4 | 4 | 9 | 13:29 | -16 | 16 | |
| 11. | Bethlehem | 17 | 4 | 4 | 9 | 23:43 | -20 | 16 | |
| 12. | Charleston | 17 | 3 | 7 | 7 | 16:24 | -8 | 16 | |
| 13. | Loudoun | 17 | 4 | 3 | 10 | 20:35 | -15 | 15 | |
| 14. | Charlotte Independ. | 17 | 2 | 9 | 6 | 16:25 | -9 | 15 | |
| 15. | Saint Louis FC | 17 | 3 | 3 | 11 | 15:25 | -10 | 12 | |
| 16. | Atlanta United 2 | 17 | 1 | 7 | 9 | 17:41 | -24 | 10 | |
| 17. | Hartford Athletic | 17 | 2 | 1 | 14 | 18:48 | -30 | 7 | |
| 18. | Sporting Kansas City II | 17 | 1 | 3 | 13 | 19:46 | -27 | 6 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | Phoenix Rising | 17 | 11 | 3 | 3 | 38:20 | 18 | 36 | |
| 2. | Reno | 17 | 10 | 2 | 5 | 33:25 | 8 | 32 | |
| 3. | Monterey Bay | 17 | 6 | 4 | 7 | 25:24 | 1 | 22 | |
| 4. | El Paso | 17 | 5 | 6 | 6 | 14:17 | -3 | 21 | |
| 5. | Ventura County | 17 | 5 | 6 | 6 | 26:34 | -8 | 21 | |
| 6. | Sacramento Republic | 17 | 6 | 2 | 9 | 19:25 | -6 | 20 | |
| 7. | New Mexico | 17 | 5 | 5 | 7 | 27:33 | -6 | 20 | |
| 8. | Orange County SC | 17 | 5 | 5 | 7 | 20:26 | -6 | 20 | |
| 9. | Portland Timbers 2 | 17 | 5 | 4 | 8 | 31:35 | -4 | 19 | |
| 10. | Real Monarchs | 17 | 5 | 4 | 8 | 24:31 | -7 | 19 | |
| 11. | Oklahoma City Energy | 17 | 4 | 5 | 8 | 24:33 | -9 | 17 | |
| 12. | Austin Bold | 17 | 3 | 6 | 8 | 18:32 | -14 | 15 | |
| 13. | FC Tulsa | 17 | 2 | 7 | 8 | 20:41 | -21 | 13 | |
| 14. | San Antonio | 17 | 3 | 3 | 11 | 25:37 | -12 | 12 | |
| 15. | Rio Grande | 17 | 2 | 5 | 10 | 24:42 | -18 | 11 | |
| 16. | Las Vegas Lights | 17 | 2 | 4 | 11 | 15:39 | -24 | 10 | |
| 17. | Tacoma Defiance | 17 | 1 | 4 | 12 | 12:51 | -39 | 7 | |
| 18. | Colorado Springs | 17 | 1 | 3 | 13 | 12:41 | -29 | 6 |