| Division | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| # | Northeast Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | New York City II | 12 | 5 | 2 | 1 | 4 | 25:21 | 20 | |
| 2. | Rochester New York | 12 | 5 | 2 | 0 | 5 | 15:16 | 19 | |
| 3. | Toronto FC II | 12 | 6 | 0 | 1 | 5 | 21:19 | 19 | |
| 4. | Philadelphia Union II | 12 | 5 | 1 | 1 | 5 | 19:22 | 18 | |
| 5. | New England Revolution II | 12 | 2 | 1 | 3 | 6 | 12:24 | 11 | |
| # | Central Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Columbus Crew 2 | 12 | 5 | 2 | 3 | 2 | 18:12 | 22 | |
| 2. | Inter Miami II | 12 | 3 | 1 | 2 | 6 | 14:33 | 13 | |
| 3. | Chicago Fire II | 12 | 3 | 1 | 1 | 7 | 18:22 | 12 | |
| 4. | Orlando City B | 12 | 3 | 0 | 0 | 9 | 14:31 | 9 | |
| 5. | Cincinnati 2 | 12 | 2 | 1 | 0 | 9 | 16:41 | 8 | |
| # | Pacific Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Town FC | 12 | 7 | 1 | 0 | 4 | 22:18 | 23 | |
| 2. | Tacoma Defiance | 12 | 5 | 3 | 0 | 4 | 23:14 | 21 | |
| 3. | Vancouver Whitecaps 2 | 12 | 3 | 2 | 2 | 5 | 15:20 | 15 | |
| 4. | Portland Timbers 2 | 12 | 1 | 1 | 0 | 10 | 11:33 | 5 | |
| 5. | Real Monarchs | 12 | 0 | 1 | 2 | 9 | 9:34 | 4 | |
| # | Frontier Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | St. Louis City 2 | 12 | 8 | 1 | 0 | 3 | 30:19 | 26 | |
| 2. | North Texas | 12 | 5 | 2 | 1 | 4 | 18:17 | 20 | |
| 3. | Houston Dynamo 2 | 12 | 4 | 2 | 2 | 4 | 17:18 | 18 | |
| 4. | Sporting Kansas City II | 12 | 5 | 1 | 1 | 5 | 12:16 | 18 | |
| 5. | Minnesota 2 | 12 | 4 | 2 | 1 | 5 | 22:23 | 17 | |
| 6. | Colorado Rapids 2 | 12 | 3 | 1 | 0 | 8 | 14:37 | 11 | |
| # | Eastern Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Columbus Crew 2 | 12 | 5 | 2 | 3 | 2 | 18:12 | 22 | |
| 2. | New York City II | 12 | 5 | 2 | 1 | 4 | 25:21 | 20 | |
| 3. | Rochester New York | 12 | 5 | 2 | 0 | 5 | 15:16 | 19 | |
| 4. | Toronto FC II | 12 | 6 | 0 | 1 | 5 | 21:19 | 19 | |
| 5. | Philadelphia Union II | 12 | 5 | 1 | 1 | 5 | 19:22 | 18 | |
| 6. | Inter Miami II | 12 | 3 | 1 | 2 | 6 | 14:33 | 13 | |
| 7. | Chicago Fire II | 12 | 3 | 1 | 1 | 7 | 18:22 | 12 | |
| 8. | New England Revolution II | 12 | 2 | 1 | 3 | 6 | 12:24 | 11 | |
| 9. | Orlando City B | 12 | 3 | 0 | 0 | 9 | 14:31 | 9 | |
| 10. | Cincinnati 2 | 12 | 2 | 1 | 0 | 9 | 16:41 | 8 | |
| # | Western Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | St. Louis City 2 | 12 | 8 | 1 | 0 | 3 | 30:19 | 26 | |
| 2. | Town FC | 12 | 7 | 1 | 0 | 4 | 22:18 | 23 | |
| 3. | Tacoma Defiance | 12 | 5 | 3 | 0 | 4 | 23:14 | 21 | |
| 4. | North Texas | 12 | 5 | 2 | 1 | 4 | 18:17 | 20 | |
| 5. | Houston Dynamo 2 | 12 | 4 | 2 | 2 | 4 | 17:18 | 18 | |
| 6. | Sporting Kansas City II | 12 | 5 | 1 | 1 | 5 | 12:16 | 18 | |
| 7. | Minnesota 2 | 12 | 4 | 2 | 1 | 5 | 22:23 | 17 | |
| 8. | Vancouver Whitecaps 2 | 12 | 3 | 2 | 2 | 5 | 15:20 | 15 | |
| 9. | Colorado Rapids 2 | 12 | 3 | 1 | 0 | 8 | 14:37 | 11 | |
| 10. | Portland Timbers 2 | 12 | 1 | 1 | 0 | 10 | 11:33 | 5 | |
| 11. | Real Monarchs | 12 | 0 | 1 | 2 | 9 | 9:34 | 4 | |