| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Charleston | 8 | 5 | 3 | 0 | 18:7 | 11 | 18 | |
| 2. | Louisville City | 6 | 5 | 0 | 1 | 17:7 | 10 | 15 | |
| 3. | Detroit | 5 | 5 | 0 | 0 | 10:4 | 6 | 15 | |
| 4. | Birmingham | 6 | 3 | 1 | 2 | 6:8 | -2 | 10 | |
| 5. | Tampa Bay | 6 | 2 | 4 | 0 | 13:7 | 6 | 10 | |
| 6. | Hartford Athletic | 5 | 3 | 0 | 2 | 7:7 | 0 | 9 | |
| 7. | Loudoun | 6 | 2 | 1 | 3 | 9:10 | -1 | 7 | |
| 8. | Rhode Island | 7 | 1 | 4 | 2 | 7:11 | -4 | 7 | |
| 9. | North Carolina | 7 | 1 | 3 | 3 | 7:10 | -3 | 6 | |
| 10. | Pittsburgh | 6 | 1 | 2 | 3 | 4:7 | -3 | 5 | |
| 11. | Indy Eleven | 7 | 1 | 2 | 4 | 11:16 | -5 | 5 | |
| 12. | Miami FC | 7 | 1 | 1 | 5 | 9:14 | -5 | 4 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | Sacramento Republic | 7 | 4 | 3 | 0 | 10:4 | 6 | 15 | |
| 2. | Monterey Bay | 7 | 4 | 2 | 1 | 11:7 | 4 | 14 | |
| 3. | New Mexico | 6 | 4 | 1 | 1 | 9:9 | 0 | 13 | |
| 4. | San Antonio | 7 | 3 | 3 | 1 | 11:7 | 4 | 12 | |
| 5. | Orange County SC | 7 | 3 | 3 | 1 | 9:6 | 3 | 12 | |
| 6. | Phoenix Rising | 8 | 3 | 1 | 4 | 9:10 | -1 | 10 | |
| 7. | Las Vegas Lights | 7 | 3 | 0 | 4 | 9:10 | -1 | 9 | |
| 8. | Oakland Roots | 7 | 2 | 1 | 4 | 7:12 | -5 | 7 | |
| 9. | FC Tulsa | 5 | 1 | 2 | 2 | 8:10 | -2 | 5 | |
| 10. | Memphis | 6 | 1 | 0 | 5 | 5:10 | -5 | 3 | |
| 11. | El Paso | 7 | 0 | 2 | 5 | 6:11 | -5 | 2 | |
| 12. | Colorado Springs | 6 | 0 | 1 | 5 | 2:10 | -8 | 1 |