| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Louisville City | 3 | 3 | 0 | 0 | 13:4 | 9 | 9 | |
| 2. | Detroit | 3 | 3 | 0 | 0 | 6:2 | 4 | 9 | |
| 3. | Charleston | 2 | 2 | 0 | 0 | 7:2 | 5 | 6 | |
| 4. | Loudoun | 3 | 2 | 0 | 1 | 5:3 | 2 | 6 | |
| 5. | Hartford Athletic | 3 | 2 | 0 | 1 | 5:5 | 0 | 6 | |
| 6. | North Carolina | 5 | 1 | 3 | 1 | 6:6 | 0 | 6 | |
| 7. | Tampa Bay | 3 | 1 | 2 | 0 | 7:4 | 3 | 5 | |
| 8. | Birmingham | 1 | 1 | 0 | 0 | 3:1 | 2 | 3 | |
| 9. | Miami FC | 5 | 1 | 0 | 4 | 5:9 | -4 | 3 | |
| 10. | Rhode Island | 4 | 0 | 3 | 1 | 2:4 | -2 | 3 | |
| 11. | Pittsburgh | 2 | 0 | 1 | 1 | 0:2 | -2 | 1 | |
| 12. | Indy Eleven | 3 | 0 | 1 | 2 | 4:7 | -3 | 1 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | San Antonio | 4 | 2 | 2 | 0 | 6:3 | 3 | 8 | |
| 2. | Monterey Bay | 3 | 2 | 1 | 0 | 6:3 | 3 | 7 | |
| 3. | New Mexico | 2 | 2 | 0 | 0 | 4:2 | 2 | 6 | |
| 4. | Las Vegas Lights | 4 | 2 | 0 | 2 | 4:5 | -1 | 6 | |
| 5. | Phoenix Rising | 5 | 2 | 0 | 3 | 3:5 | -2 | 6 | |
| 6. | Sacramento Republic | 3 | 1 | 2 | 0 | 4:3 | 1 | 5 | |
| 7. | Orange County SC | 3 | 1 | 1 | 1 | 3:4 | -1 | 4 | |
| 8. | Oakland Roots | 4 | 1 | 1 | 2 | 3:6 | -3 | 4 | |
| 9. | Memphis | 4 | 1 | 0 | 3 | 4:7 | -3 | 3 | |
| 10. | FC Tulsa | 2 | 0 | 1 | 1 | 4:7 | -3 | 1 | |
| 11. | El Paso | 4 | 0 | 1 | 3 | 3:6 | -3 | 1 | |
| 12. | Colorado Springs | 3 | 0 | 1 | 2 | 2:5 | -3 | 1 |