| Division | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| # | Northeast Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Philadelphia Union II | 5 | 3 | 2 | 0 | 0 | 8:4 | 13 | |
| 2. | Chicago Fire II | 4 | 3 | 1 | 0 | 0 | 7:2 | 11 | |
| 3. | Cincinnati 2 | 5 | 3 | 0 | 1 | 1 | 10:7 | 10 | |
| 4. | New England Revolution II | 3 | 3 | 0 | 0 | 0 | 8:4 | 9 | |
| 5. | New York City II | 4 | 1 | 1 | 0 | 2 | 8:7 | 5 | |
| 6. | Toronto FC II | 3 | 1 | 0 | 2 | 0 | 4:3 | 5 | |
| 7. | New York Red Bulls 2 | 3 | 0 | 2 | 0 | 1 | 8:10 | 4 | |
| 8. | Columbus Crew 2 | 3 | 1 | 0 | 0 | 2 | 2:5 | 3 | |
| # | Southeast Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Chattanooga | 5 | 3 | 2 | 0 | 0 | 14:5 | 13 | |
| 2. | Atlanta United 2 | 6 | 2 | 1 | 0 | 3 | 8:12 | 8 | |
| 3. | Inter Miami II | 3 | 2 | 0 | 0 | 1 | 6:5 | 6 | |
| 4. | Crown Legacy | 3 | 0 | 1 | 1 | 1 | 4:6 | 3 | |
| 5. | Huntsville | 4 | 0 | 0 | 2 | 2 | 5:7 | 2 | |
| 6. | Carolina Core | 0 | 0 | 0 | 0 | 0 | 0:0 | 0 | |
| 7. | Orlando City B | 2 | 0 | 0 | 0 | 2 | 1:4 | 0 | |
| # | Pacific Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Los Angeles FC II | 4 | 4 | 0 | 0 | 0 | 11:4 | 12 | |
| 2. | Ventura County | 4 | 3 | 1 | 0 | 0 | 10:5 | 11 | |
| 3. | Tacoma Defiance | 4 | 3 | 1 | 0 | 0 | 9:4 | 11 | |
| 4. | Real Monarchs | 5 | 2 | 1 | 0 | 2 | 5:5 | 8 | |
| 5. | Portland Timbers 2 | 4 | 2 | 0 | 0 | 2 | 8:7 | 6 | |
| 6. | Vancouver Whitecaps 2 | 2 | 1 | 0 | 1 | 0 | 7:5 | 4 | |
| 7. | Town FC | 2 | 0 | 1 | 1 | 0 | 1:1 | 3 | |
| # | Frontier Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | North Texas | 4 | 1 | 2 | 1 | 0 | 5:4 | 8 | |
| 2. | Sporting Kansas City II | 5 | 2 | 0 | 0 | 3 | 10:8 | 6 | |
| 3. | St. Louis City 2 | 4 | 1 | 1 | 1 | 1 | 5:4 | 6 | |
| 4. | Austin FC II | 3 | 0 | 0 | 1 | 2 | 3:6 | 1 | |
| 5. | Colorado Rapids 2 | 4 | 0 | 0 | 1 | 3 | 5:10 | 1 | |
| 6. | Minnesota 2 | 1 | 0 | 0 | 0 | 1 | 1:2 | 0 | |
| 7. | Houston Dynamo 2 | 2 | 0 | 0 | 0 | 2 | 1:3 | 0 | |
| # | Eastern Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Chattanooga | 5 | 3 | 2 | 0 | 0 | 14:5 | 13 | |
| 2. | Philadelphia Union II | 5 | 3 | 2 | 0 | 0 | 8:4 | 13 | |
| 3. | Chicago Fire II | 4 | 3 | 1 | 0 | 0 | 7:2 | 11 | |
| 4. | Cincinnati 2 | 5 | 3 | 0 | 1 | 1 | 10:7 | 10 | |
| 5. | New England Revolution II | 3 | 3 | 0 | 0 | 0 | 8:4 | 9 | |
| 6. | Atlanta United 2 | 6 | 2 | 1 | 0 | 3 | 8:12 | 8 | |
| 7. | Inter Miami II | 3 | 2 | 0 | 0 | 1 | 6:5 | 6 | |
| 8. | New York City II | 4 | 1 | 1 | 0 | 2 | 8:7 | 5 | |
| 9. | Toronto FC II | 3 | 1 | 0 | 2 | 0 | 4:3 | 5 | |
| 10. | New York Red Bulls 2 | 3 | 0 | 2 | 0 | 1 | 8:10 | 4 | |
| 11. | Crown Legacy | 3 | 0 | 1 | 1 | 1 | 4:6 | 3 | |
| 12. | Columbus Crew 2 | 3 | 1 | 0 | 0 | 2 | 2:5 | 3 | |
| 13. | Huntsville | 4 | 0 | 0 | 2 | 2 | 5:7 | 2 | |
| 14. | Carolina Core | 0 | 0 | 0 | 0 | 0 | 0:0 | 0 | |
| 15. | Orlando City B | 2 | 0 | 0 | 0 | 2 | 1:4 | 0 | |
| # | Western Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Los Angeles FC II | 4 | 4 | 0 | 0 | 0 | 11:4 | 12 | |
| 2. | Ventura County | 4 | 3 | 1 | 0 | 0 | 10:5 | 11 | |
| 3. | Tacoma Defiance | 4 | 3 | 1 | 0 | 0 | 9:4 | 11 | |
| 4. | North Texas | 4 | 1 | 2 | 1 | 0 | 5:4 | 8 | |
| 5. | Real Monarchs | 5 | 2 | 1 | 0 | 2 | 5:5 | 8 | |
| 6. | Sporting Kansas City II | 5 | 2 | 0 | 0 | 3 | 10:8 | 6 | |
| 7. | Portland Timbers 2 | 4 | 2 | 0 | 0 | 2 | 8:7 | 6 | |
| 8. | St. Louis City 2 | 4 | 1 | 1 | 1 | 1 | 5:4 | 6 | |
| 9. | Vancouver Whitecaps 2 | 2 | 1 | 0 | 1 | 0 | 7:5 | 4 | |
| 10. | Town FC | 2 | 0 | 1 | 1 | 0 | 1:1 | 3 | |
| 11. | Austin FC II | 3 | 0 | 0 | 1 | 2 | 3:6 | 1 | |
| 12. | Colorado Rapids 2 | 4 | 0 | 0 | 1 | 3 | 5:10 | 1 | |
| 13. | Minnesota 2 | 1 | 0 | 0 | 0 | 1 | 1:2 | 0 | |
| 14. | Houston Dynamo 2 | 2 | 0 | 0 | 0 | 2 | 1:3 | 0 | |